pkh67 labeled sevs Search Results


vectashield antifade mounting medium with dapi  (Vector Laboratories)
98
Vector Laboratories vectashield antifade mounting medium with dapi
Vectashield Antifade Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkh67+labeled+sevs/custom%40h-1200%4034484864?v=Vector+Laboratories
Average 98 stars, based on 1 article reviews
vectashield antifade mounting medium with dapi - by Bioz Stars, 2026-07
98/100 stars
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100 kda filter  (Sartorius AG)
95
Sartorius AG 100 kda filter
100 Kda Filter, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkh67+labeled+sevs/pmc06158211-240-32-34?v=Sartorius+AG
Average 95 stars, based on 1 article reviews
100 kda filter - by Bioz Stars, 2026-07
95/100 stars
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pkh67  (Merck & Co)
86
Merck & Co pkh67
( A ) Schematic representation of the experimental approach used to test whether senescent cell–derived small extracellular vesicles and particles (EVPs) can be taken up by proliferating cells. Proliferating IMR90 cells were treated with <t>PKH67,</t> a green fluorescent lipophilic membrane dye, alone or with PKH67-labeled EVPs isolated from senescent cells. ( B ) Representative immunofluorescence images of proliferating cells treated with or without PKH67-labeled sEVP fractions from senescent cells, using different sEVP volumes while keeping the PKH67 lipid dye concentration unchanged. Boxes indicate expanded region and arrows indicate PKH67 puncta in recipient cells. ( C ) Quantification of internalized PKH67-labeled sEVPs by proliferating cells. Data are presented as mean ± SD (n=3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.023, **p=0.0010, ****p<0.0001. ( D ) Schematic representation of the experimental setup: proliferating IMR90 cells were treated with no sEVPs or sEVPs secreted by proliferating cells treated with DMSO, or senescent cells treated with the inhibitor of CCF formation, MDM2 inhibitor RG7388 (MDM2i) or DMSO vehicle. ( E ) Quantification of cGAMP concentration in proliferating cells treated with no sEVPs or sEVPs (40 μL) from proliferating cells treated with DMSO, or senescent cells treated with MDM2i or DMSO vehicle. sEVPs were isolated from equivalent numbers of cells for each condition and resuspended in identical buffer volumes. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.011, **p=0.0094.
Pkh67, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkh67+labeled+sevs/bio_rxiv__64898__2025__12__12__694055-260-5-7?v=Merck+%26+Co
Average 86 stars, based on 1 article reviews
pkh67 - by Bioz Stars, 2026-07
86/100 stars
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24-well plates  (Corning Life Sciences)
90
Corning Life Sciences 24-well plates
( A ) Schematic representation of the experimental approach used to test whether senescent cell–derived small extracellular vesicles and particles (EVPs) can be taken up by proliferating cells. Proliferating IMR90 cells were treated with <t>PKH67,</t> a green fluorescent lipophilic membrane dye, alone or with PKH67-labeled EVPs isolated from senescent cells. ( B ) Representative immunofluorescence images of proliferating cells treated with or without PKH67-labeled sEVP fractions from senescent cells, using different sEVP volumes while keeping the PKH67 lipid dye concentration unchanged. Boxes indicate expanded region and arrows indicate PKH67 puncta in recipient cells. ( C ) Quantification of internalized PKH67-labeled sEVPs by proliferating cells. Data are presented as mean ± SD (n=3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.023, **p=0.0010, ****p<0.0001. ( D ) Schematic representation of the experimental setup: proliferating IMR90 cells were treated with no sEVPs or sEVPs secreted by proliferating cells treated with DMSO, or senescent cells treated with the inhibitor of CCF formation, MDM2 inhibitor RG7388 (MDM2i) or DMSO vehicle. ( E ) Quantification of cGAMP concentration in proliferating cells treated with no sEVPs or sEVPs (40 μL) from proliferating cells treated with DMSO, or senescent cells treated with MDM2i or DMSO vehicle. sEVPs were isolated from equivalent numbers of cells for each condition and resuspended in identical buffer volumes. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.011, **p=0.0094.
24 Well Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkh67+labeled+sevs/ppr0385086-115-13-16?v=Corning+Life+Sciences
Average 90 stars, based on 1 article reviews
24-well plates - by Bioz Stars, 2026-07
90/100 stars
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egm-2 exo-depleted medium  (Lonza)
90
Lonza egm-2 exo-depleted medium
( A ) Schematic representation of the experimental approach used to test whether senescent cell–derived small extracellular vesicles and particles (EVPs) can be taken up by proliferating cells. Proliferating IMR90 cells were treated with <t>PKH67,</t> a green fluorescent lipophilic membrane dye, alone or with PKH67-labeled EVPs isolated from senescent cells. ( B ) Representative immunofluorescence images of proliferating cells treated with or without PKH67-labeled sEVP fractions from senescent cells, using different sEVP volumes while keeping the PKH67 lipid dye concentration unchanged. Boxes indicate expanded region and arrows indicate PKH67 puncta in recipient cells. ( C ) Quantification of internalized PKH67-labeled sEVPs by proliferating cells. Data are presented as mean ± SD (n=3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.023, **p=0.0010, ****p<0.0001. ( D ) Schematic representation of the experimental setup: proliferating IMR90 cells were treated with no sEVPs or sEVPs secreted by proliferating cells treated with DMSO, or senescent cells treated with the inhibitor of CCF formation, MDM2 inhibitor RG7388 (MDM2i) or DMSO vehicle. ( E ) Quantification of cGAMP concentration in proliferating cells treated with no sEVPs or sEVPs (40 μL) from proliferating cells treated with DMSO, or senescent cells treated with MDM2i or DMSO vehicle. sEVPs were isolated from equivalent numbers of cells for each condition and resuspended in identical buffer volumes. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.011, **p=0.0094.
Egm 2 Exo Depleted Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkh67+labeled+sevs/pmc09010519-388-35-36?v=Lonza
Average 90 stars, based on 1 article reviews
egm-2 exo-depleted medium - by Bioz Stars, 2026-07
90/100 stars
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wt mouse  (Miltenyi Biotec)
96
Miltenyi Biotec wt mouse
( A ) Schematic representation of the experimental approach used to test whether senescent cell–derived small extracellular vesicles and particles (EVPs) can be taken up by proliferating cells. Proliferating IMR90 cells were treated with <t>PKH67,</t> a green fluorescent lipophilic membrane dye, alone or with PKH67-labeled EVPs isolated from senescent cells. ( B ) Representative immunofluorescence images of proliferating cells treated with or without PKH67-labeled sEVP fractions from senescent cells, using different sEVP volumes while keeping the PKH67 lipid dye concentration unchanged. Boxes indicate expanded region and arrows indicate PKH67 puncta in recipient cells. ( C ) Quantification of internalized PKH67-labeled sEVPs by proliferating cells. Data are presented as mean ± SD (n=3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.023, **p=0.0010, ****p<0.0001. ( D ) Schematic representation of the experimental setup: proliferating IMR90 cells were treated with no sEVPs or sEVPs secreted by proliferating cells treated with DMSO, or senescent cells treated with the inhibitor of CCF formation, MDM2 inhibitor RG7388 (MDM2i) or DMSO vehicle. ( E ) Quantification of cGAMP concentration in proliferating cells treated with no sEVPs or sEVPs (40 μL) from proliferating cells treated with DMSO, or senescent cells treated with MDM2i or DMSO vehicle. sEVPs were isolated from equivalent numbers of cells for each condition and resuspended in identical buffer volumes. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.011, **p=0.0094.
Wt Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkh67+labeled+sevs/pmc11443396__2023_284145_LI_SUPPL-27-14-21?v=Miltenyi+Biotec
Average 96 stars, based on 1 article reviews
wt mouse - by Bioz Stars, 2026-07
96/100 stars
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cd117 microbeads  (Miltenyi Biotec)
96
Miltenyi Biotec cd117 microbeads
( A ) Schematic representation of the experimental approach used to test whether senescent cell–derived small extracellular vesicles and particles (EVPs) can be taken up by proliferating cells. Proliferating IMR90 cells were treated with <t>PKH67,</t> a green fluorescent lipophilic membrane dye, alone or with PKH67-labeled EVPs isolated from senescent cells. ( B ) Representative immunofluorescence images of proliferating cells treated with or without PKH67-labeled sEVP fractions from senescent cells, using different sEVP volumes while keeping the PKH67 lipid dye concentration unchanged. Boxes indicate expanded region and arrows indicate PKH67 puncta in recipient cells. ( C ) Quantification of internalized PKH67-labeled sEVPs by proliferating cells. Data are presented as mean ± SD (n=3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.023, **p=0.0010, ****p<0.0001. ( D ) Schematic representation of the experimental setup: proliferating IMR90 cells were treated with no sEVPs or sEVPs secreted by proliferating cells treated with DMSO, or senescent cells treated with the inhibitor of CCF formation, MDM2 inhibitor RG7388 (MDM2i) or DMSO vehicle. ( E ) Quantification of cGAMP concentration in proliferating cells treated with no sEVPs or sEVPs (40 μL) from proliferating cells treated with DMSO, or senescent cells treated with MDM2i or DMSO vehicle. sEVPs were isolated from equivalent numbers of cells for each condition and resuspended in identical buffer volumes. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.011, **p=0.0094.
Cd117 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkh67+labeled+sevs/pmc11443396__2023_284145_LI_SUPPL-27-19-21?v=Miltenyi+Biotec
Average 96 stars, based on 1 article reviews
cd117 microbeads - by Bioz Stars, 2026-07
96/100 stars
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olympus ixplore pro microscope  (Olympus)
97
Olympus olympus ixplore pro microscope
( A ) Schematic representation of the experimental approach used to test whether senescent cell–derived small extracellular vesicles and particles (EVPs) can be taken up by proliferating cells. Proliferating IMR90 cells were treated with <t>PKH67,</t> a green fluorescent lipophilic membrane dye, alone or with PKH67-labeled EVPs isolated from senescent cells. ( B ) Representative immunofluorescence images of proliferating cells treated with or without PKH67-labeled sEVP fractions from senescent cells, using different sEVP volumes while keeping the PKH67 lipid dye concentration unchanged. Boxes indicate expanded region and arrows indicate PKH67 puncta in recipient cells. ( C ) Quantification of internalized PKH67-labeled sEVPs by proliferating cells. Data are presented as mean ± SD (n=3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.023, **p=0.0010, ****p<0.0001. ( D ) Schematic representation of the experimental setup: proliferating IMR90 cells were treated with no sEVPs or sEVPs secreted by proliferating cells treated with DMSO, or senescent cells treated with the inhibitor of CCF formation, MDM2 inhibitor RG7388 (MDM2i) or DMSO vehicle. ( E ) Quantification of cGAMP concentration in proliferating cells treated with no sEVPs or sEVPs (40 μL) from proliferating cells treated with DMSO, or senescent cells treated with MDM2i or DMSO vehicle. sEVPs were isolated from equivalent numbers of cells for each condition and resuspended in identical buffer volumes. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.011, **p=0.0094.
Olympus Ixplore Pro Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkh67+labeled+sevs/pmc12492563-71-45-45?v=Olympus
Average 97 stars, based on 1 article reviews
olympus ixplore pro microscope - by Bioz Stars, 2026-07
97/100 stars
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nanosight tracking analysis  (NanoSight ltd)
90
NanoSight ltd nanosight tracking analysis
( A ) Schematic representation of the experimental approach used to test whether senescent cell–derived small extracellular vesicles and particles (EVPs) can be taken up by proliferating cells. Proliferating IMR90 cells were treated with <t>PKH67,</t> a green fluorescent lipophilic membrane dye, alone or with PKH67-labeled EVPs isolated from senescent cells. ( B ) Representative immunofluorescence images of proliferating cells treated with or without PKH67-labeled sEVP fractions from senescent cells, using different sEVP volumes while keeping the PKH67 lipid dye concentration unchanged. Boxes indicate expanded region and arrows indicate PKH67 puncta in recipient cells. ( C ) Quantification of internalized PKH67-labeled sEVPs by proliferating cells. Data are presented as mean ± SD (n=3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.023, **p=0.0010, ****p<0.0001. ( D ) Schematic representation of the experimental setup: proliferating IMR90 cells were treated with no sEVPs or sEVPs secreted by proliferating cells treated with DMSO, or senescent cells treated with the inhibitor of CCF formation, MDM2 inhibitor RG7388 (MDM2i) or DMSO vehicle. ( E ) Quantification of cGAMP concentration in proliferating cells treated with no sEVPs or sEVPs (40 μL) from proliferating cells treated with DMSO, or senescent cells treated with MDM2i or DMSO vehicle. sEVPs were isolated from equivalent numbers of cells for each condition and resuspended in identical buffer volumes. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.011, **p=0.0094.
Nanosight Tracking Analysis, supplied by NanoSight ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkh67+labeled+sevs/pmc11426245__thnov14p5926s1-166-66-66?v=NanoSight+ltd
Average 90 stars, based on 1 article reviews
nanosight tracking analysis - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


( A ) Schematic representation of the experimental approach used to test whether senescent cell–derived small extracellular vesicles and particles (EVPs) can be taken up by proliferating cells. Proliferating IMR90 cells were treated with PKH67, a green fluorescent lipophilic membrane dye, alone or with PKH67-labeled EVPs isolated from senescent cells. ( B ) Representative immunofluorescence images of proliferating cells treated with or without PKH67-labeled sEVP fractions from senescent cells, using different sEVP volumes while keeping the PKH67 lipid dye concentration unchanged. Boxes indicate expanded region and arrows indicate PKH67 puncta in recipient cells. ( C ) Quantification of internalized PKH67-labeled sEVPs by proliferating cells. Data are presented as mean ± SD (n=3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.023, **p=0.0010, ****p<0.0001. ( D ) Schematic representation of the experimental setup: proliferating IMR90 cells were treated with no sEVPs or sEVPs secreted by proliferating cells treated with DMSO, or senescent cells treated with the inhibitor of CCF formation, MDM2 inhibitor RG7388 (MDM2i) or DMSO vehicle. ( E ) Quantification of cGAMP concentration in proliferating cells treated with no sEVPs or sEVPs (40 μL) from proliferating cells treated with DMSO, or senescent cells treated with MDM2i or DMSO vehicle. sEVPs were isolated from equivalent numbers of cells for each condition and resuspended in identical buffer volumes. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.011, **p=0.0094.

Journal: bioRxiv

Article Title: Senescent cells secrete chromatin components via senescence-associated extracellular particles

doi: 10.64898/2025.12.12.694055

Figure Lengend Snippet: ( A ) Schematic representation of the experimental approach used to test whether senescent cell–derived small extracellular vesicles and particles (EVPs) can be taken up by proliferating cells. Proliferating IMR90 cells were treated with PKH67, a green fluorescent lipophilic membrane dye, alone or with PKH67-labeled EVPs isolated from senescent cells. ( B ) Representative immunofluorescence images of proliferating cells treated with or without PKH67-labeled sEVP fractions from senescent cells, using different sEVP volumes while keeping the PKH67 lipid dye concentration unchanged. Boxes indicate expanded region and arrows indicate PKH67 puncta in recipient cells. ( C ) Quantification of internalized PKH67-labeled sEVPs by proliferating cells. Data are presented as mean ± SD (n=3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.023, **p=0.0010, ****p<0.0001. ( D ) Schematic representation of the experimental setup: proliferating IMR90 cells were treated with no sEVPs or sEVPs secreted by proliferating cells treated with DMSO, or senescent cells treated with the inhibitor of CCF formation, MDM2 inhibitor RG7388 (MDM2i) or DMSO vehicle. ( E ) Quantification of cGAMP concentration in proliferating cells treated with no sEVPs or sEVPs (40 μL) from proliferating cells treated with DMSO, or senescent cells treated with MDM2i or DMSO vehicle. sEVPs were isolated from equivalent numbers of cells for each condition and resuspended in identical buffer volumes. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.011, **p=0.0094.

Article Snippet: sEVs were fluorescently labeled with PKH67 (MINI67-1KT, Merck) following the manufacturer’s guidelines with modifications to optimize specificity.

Techniques: Derivative Assay, Membrane, Labeling, Isolation, Immunofluorescence, Concentration Assay