pkh67 labeled sevs Search Results


pkh67-labeled sevs  (Procell Inc)
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Procell Inc pkh67-labeled sevs
Characterization of hUC-MSC-sEVs. ( A ) Schematic diagram of the extraction process of hUC-MSC-sEVs. ( B ) Under the electron microscope, the hUC-MSC-sEVs show a circular bilayer structure with a diameter of about 100 nm. ( C ) The results of nFCM showed that the diameter of hUC-MSC-sEVs was about 80.48 nm. ( D ) Proportional relationship among original supernatant volume, quantification of cells and vesicles particles, and amount of protein extracted from hUC-MSC-sEVs. ( E ) The surface markers of hUC-MSC-sEVs were identified by nFCM. CD9, CD63, and CD81 were found to be positive in hUC-MSC-sEVs. ( F ) hUC-MSC-sEVs’ internalization to chondrocytes. hUC-MSC-sEVs (labeled with <t>PKH67</t> dye, green) and chondrocytes (nuclei were stained with DAPI) were co-incubated for 12 h, respectively. In the control group, PKH67 dye was co-incubated with chondrocytes for 12 h, respectively. Representative fluorescence images are shown above (scale bar = 100 μm; scale bar in magnification = 50 μm).
Pkh67 Labeled Sevs, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pkh67-labeled sevs - by Bioz Stars, 2026-03
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Characterization of hUC-MSC-sEVs. ( A ) Schematic diagram of the extraction process of hUC-MSC-sEVs. ( B ) Under the electron microscope, the hUC-MSC-sEVs show a circular bilayer structure with a diameter of about 100 nm. ( C ) The results of nFCM showed that the diameter of hUC-MSC-sEVs was about 80.48 nm. ( D ) Proportional relationship among original supernatant volume, quantification of cells and vesicles particles, and amount of protein extracted from hUC-MSC-sEVs. ( E ) The surface markers of hUC-MSC-sEVs were identified by nFCM. CD9, CD63, and CD81 were found to be positive in hUC-MSC-sEVs. ( F ) hUC-MSC-sEVs’ internalization to chondrocytes. hUC-MSC-sEVs (labeled with PKH67 dye, green) and chondrocytes (nuclei were stained with DAPI) were co-incubated for 12 h, respectively. In the control group, PKH67 dye was co-incubated with chondrocytes for 12 h, respectively. Representative fluorescence images are shown above (scale bar = 100 μm; scale bar in magnification = 50 μm).

Journal: International Journal of Nanomedicine

Article Title: Comparison of Curative Effect of Human Umbilical Cord-Derived Mesenchymal Stem Cells and Their Small Extracellular Vesicles in Treating Osteoarthritis

doi: 10.2147/IJN.S336062

Figure Lengend Snippet: Characterization of hUC-MSC-sEVs. ( A ) Schematic diagram of the extraction process of hUC-MSC-sEVs. ( B ) Under the electron microscope, the hUC-MSC-sEVs show a circular bilayer structure with a diameter of about 100 nm. ( C ) The results of nFCM showed that the diameter of hUC-MSC-sEVs was about 80.48 nm. ( D ) Proportional relationship among original supernatant volume, quantification of cells and vesicles particles, and amount of protein extracted from hUC-MSC-sEVs. ( E ) The surface markers of hUC-MSC-sEVs were identified by nFCM. CD9, CD63, and CD81 were found to be positive in hUC-MSC-sEVs. ( F ) hUC-MSC-sEVs’ internalization to chondrocytes. hUC-MSC-sEVs (labeled with PKH67 dye, green) and chondrocytes (nuclei were stained with DAPI) were co-incubated for 12 h, respectively. In the control group, PKH67 dye was co-incubated with chondrocytes for 12 h, respectively. Representative fluorescence images are shown above (scale bar = 100 μm; scale bar in magnification = 50 μm).

Article Snippet: Subsequently, PKH67-labeled sEVs were co-cultured with chondrocytes (CP-R092, Procell Life Sci & Tech Co. Ltd., Wuhan, China) for 12 h, and the internalization of sEVs was evaluated using fluorescence microscopy (Leica DMi8 S, Germany).

Techniques: Microscopy, Labeling, Staining, Incubation, Fluorescence